Chapter 116: Vortex Bones To Shake Well

And just when I thought I would be doing the same thing throughout my FYP, something new pops out.

A while back, I had subcultured six plates from my collection of isolated fungi, so we could prepare their DNA for identification later on, according to a method used by some company that is supposed to be more efficient than a pulse-field method (science talk), based on the limits of their own database.

What I didn't know is that we were going to do the preparation so soon.

Scraping the plates seemed simple enough on the surface... except my own plates happened to be pretty old, and the older it was, the harder it was scrape it off without breaking the celloplane membrane used. Even to the point of bending the inoculating loop, it still wouldn't budge. Yeast, of which I had one sample of was, was known to be harder to pick up into the loop for a sample than others. Heh. Even at this time, I won't get to catch a break. Oh well.

Vortexing the DNA after cycles of freezing in liquid nitrogen to disrupt the sample structures so as to get the DNA was quite interesting as well. Holding the samples to be vortexed onto the machine for two cycles of five minutes and then twenty minutes had no skill involved, but it sure rattled the old bones. Pain didn't last long either.

That will be all for now.